Product Name :
SF3B3 Recombinant Rabbit Monoclonal Antibody [JG63-53]
Predicted band size :
136 kDa
Observed band size :
136 kDa
Synonyms:
KIAA0017 antibody Pre mRNA splicing factor SF3b 130 kDa subunit antibody Pre-mRNA-splicing factor SF3b 130 kDa subunit antibody RSE1 antibody SAP 130 antibody SAP130 antibody SF3b130 antibody SF3B3 antibody SF3B3_HUMAN antibody Spliceosome associated protein 130 antibody 展开
Function :
SF3B is a U2 snRNP-associated protein complex essential for spliceosome assembly. SF3B contains the spliceosomal proteins SAP 49, SAP 130 (also known as SF3B3), SAP 145 and SAP 155. SF3B3, SAP 145 and SAP 155 are present in a protein complex in HeLa nuclear extracts and associate with one another. While SF3B3 and SAP 155 interact with each other (directly or indirectly) within this complex, SAP 49 and SAP 145 are known to interact directly with each other. Unexpectedly, the SAP 49-SAP 145 protein-protein interaction requires the amino-terminus of SAP 49, which contains two RNA-recognition motifs. The observation that SAP 49 and SAP 145 interact directly with both U2 snRNP and the pre-mRNA suggests that this protein complex plays a role in tethering U2 snRNP to the branch site.
Antibody Type:
Recombinant Rabbit monoclonal Antibody
Immunogen :
Recombinant protein within Human SF3B3 aa 1061-1217 / 1217.
Species reactivity:
Human, Mouse, Rat
Verify application:
WB, IF-Cell, IHC-P, FC
Coupling :
unconjugated
Clone number:
JG63-53
RRID:
AB_3070835
Form :
Liquid
Concentration :
1ug/ul
Storage instructions:
Store at +4℃ after thawing.8-Bromo-3-chloroisoquinoline Order Aliquot store at -20℃.DOTA-tris(tBu)ester NHS ester manufacturer Avoid repeated freeze / thaw cycles.PMID:33590949
Storage buffer solution:
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Subtype:
IgG
Purification method :
Protein A affinity purified.
ELTSA :
Molecular weight :
136 kDa
Positive control :
Jurkat cell lysate, Hela cell lysate, human colon cancer tissue, human kidney tissue, mouse testis tissue, human tonsil tissue, rat brain tissue, LOVO.